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ATCC
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ATCC
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NSJ Bioreagents
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Agilent technologies
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Image Search Results
Journal: Cellular and Molecular Life Sciences
Article Title: Shared extracellular vesicle miRNA profiles of matched ductal pancreatic adenocarcinoma organoids and blood plasma samples show the power of organoid technology
doi: 10.1007/s00018-020-03703-8
Figure Lengend Snippet: PDAC cell lines produce more EVs than control pancreas ductal cells both in 2D and 3D cultures. a The morphology of different cell lines in 2D and their colonies in 3D. Note that HPDEC is an immortalized pancreatic ductal control cell line. b CD63 + /CD81 + extracellular vesicle release of the indicated cell lines, measured by antibody-coated beads and flow cytometry (normalized to 10 6 cells). The numbers after ‘D’ mean 2D or 3D cultures. c – d The distribution of particle size from 3D cultures of PDAC cell lines, determined by NTA. Note that cells, cell debris and large EVs were removed by serial centrifugation at 300 g and 2000 g. e Quantification of the particle concentration from 2 and 3D cultures ( n = 4, data are normalized to 10 6 cells). f miRNA levels in EV preparates isolated from Panc10.05 cells with serial centrifugation and ultracentrifugation (UC) or by a mixture of anti-CD81 and anti-CD63-coated beads (beads), measured by miRNA assays. Note that the empty, serum-free medium was used as control (Cells-). Scale bars: 50 µm ( a , upper panels) or 10 µm ( a , lower panels)
Article Snippet: We used
Techniques: Control, Flow Cytometry, Centrifugation, Concentration Assay, Isolation
Journal: Cellular and Molecular Life Sciences
Article Title: Shared extracellular vesicle miRNA profiles of matched ductal pancreatic adenocarcinoma organoids and blood plasma samples show the power of organoid technology
doi: 10.1007/s00018-020-03703-8
Figure Lengend Snippet: miRNAs with a stable level across PDAC cell line-derived samples with two different algorithms
Article Snippet: We used
Techniques:
Journal: Cellular and Molecular Life Sciences
Article Title: Shared extracellular vesicle miRNA profiles of matched ductal pancreatic adenocarcinoma organoids and blood plasma samples show the power of organoid technology
doi: 10.1007/s00018-020-03703-8
Figure Lengend Snippet: Effect of 3D culturing on the miRNA cargo of PDAC cell-derived EVs is dependent on the matrix. a – b The morphology of the colonies in different matrices (left panels) and correlations between the miRNA levels of EV samples from 2D and 3D cultures in the indicated matrices for Panc08.13 ( a ) and Panc10.05 ( b ) cells (low-density miRNA cards). Ct values were normalized to the level of miR-19b. c The number and percentage of the overlapping and non-overlapping miRNAs of Panc08.13 (blue color) and Panc10.05 (yellow circles) cell-derived EVs cultured under the indicated conditions. Scale bars: 50 µm ( a , b )
Article Snippet: We used
Techniques: Derivative Assay, Cell Culture
Journal: Cellular and Molecular Life Sciences
Article Title: Shared extracellular vesicle miRNA profiles of matched ductal pancreatic adenocarcinoma organoids and blood plasma samples show the power of organoid technology
doi: 10.1007/s00018-020-03703-8
Figure Lengend Snippet: POCS miRNA set, present in all PDAC organoid EVs, shows a complete overlap with PDAC blood EVs and a partial overlap with PDAC cell line (Panc10.05, Panc08.13)-derived EV cargo (cultured in Matrigel)
Article Snippet: We used
Techniques: Cell Culture, Clinical Proteomics